H+ Output in Male and Female Rats as Affected by Old Age

An experiment was conducted to valuate the H+ output in rats stomachs in male and females after [1] and 8 weeks feeding period with a modified standard rodent diet. A total of 56 Albino strain rats (28 per sex, 20 day-old) were reared underwent a bring rein terms of lighting (half time of day) and temperature (23 ± 2°C) during trial. Starting experiments was in 8:00 AM, as animals deprived from feed only, 18 hours before starting trial. By anesthetize rats with Sodium thiopental and insert a silicon tube in stomach via duodenum, the amounts of acids were collected and measured with an automatic acid titration (TTT 80), withNa OH 0.1 N to pH=7. When significant differences (P < 0.05) were detected, the mean values were compared post-hoc by using the student t- test. Also, the correlation coefficients were calculated. Results from this study did not showed significant difference in secretion content of the basis gastric acid. In both male and female, the basal acid amount was correlated with age (R=0.4, P<0.0) and stomach weight (R=0.44, P<0.01) and the stomach/body weight was bigger in female (P<0.05) than males, but means of the body and stomach weight were more in male (P<0.05). Old age significantly increased the amount of basis gastric acid secretion in males. With regard to absence of significant difference in the mean of basal acid output between male and female rats in spite of significant differences in their body weights, selecting one of both sexes from both ages was proposed for different studies on gastric acid secretion.