Abstract :
These procedures were conducted in vitro for propagation of Jojoba. Shoot tip explants were cultured on MS, WPM and B5 media at different of salt strengths (double full strength, one and half full strength, one and quarter full strength, full strength, 3/4, 1/2, 1/4 and 1/8) free of growth regulators. Effect of MS and 1.25 WPM media supplemented with 2ip, at various concentrations of 0.0, 0.1, 0.2, 0.4, 0.8 and 1.6 mgl-1, Kin and IAA at various concentrations of 0.0, 0.5, 1.0, 1.5, 2.0 and 2.5 mgl-1 in addition to 0.170 mgl-1 ZnSo4.7H2o, 0.170 N2H2Po4, 12.0 mgl-1 Boric acid and 0.25 mgl-1 CuSo4 for each. Also the effect of 1.25 WPM supplemented with BA at concentrations of (0.0, 1.75, 3.5, 7.0 mgl-1 )in addition to 0.170 mgl-1 ZnSo4.7H2o, 0.170 N2H2Po4, 12.0 mgl-1 Boric acid and 0.25 mgl-1 CuSo were investigated, to study their effects on the growth and development of Jojoba shoots in the multiplication stage. In the rooting stage the effect of IBA at 4.9, 9.9 and 19.9 mgl-1 when it added to MS full strength and1.25 WPM full strength media was investigated. Results showed that shoot growth at the establishment stage required 1.25 strength of WPM medium or MS full strength medium( free of growth regulators) and shoot multiplication required MS strength supplemented with 0.1 2ip mgl-1+ 0.5 mgL-1 IAA+2.0 mgl-1 Kin + 0.170 mgl-1 ZnSo4.7H2o + 0.170 N2H2Po4 + 12.0 mgl-1 Boric acid + 0.25 mgl-1 CuSo4 or 1.25 full strength of WPM medium supplemented with 7.0 mgl-1 BA +0.5 mgl-1 IAA+ 0.170 mgl-1 ZnSo4.7H2o+ 0.170 N2H2Po4 + 12.0 mgl-1 Boric acid + 0.25 mgl-1 CuSo ).Rooted shoots required MS full strength with 4.9 mgl-1 IBA. Rooted shoots were acclimatized and successfully transferred to soil (peat: sand 1:2 v/v) with 60 % the surviving of plants
