Optimization of Endoglucanase Production by Aspergillus niger VTCC-F021

Among 63 Aspergillus niger strains collected at the Vietnam Type Culture Collection, the strain Aspergillus niger VTCC-F021 showed the highest endoglucanase production. The endoglucanase production by A. niger VTCC-F021 attained the maximum in CPY medium containing (w/v) 0.4% peptone, 0.1% extrat, 0.1% K2HPO4, 0.05% KCl, 0.05% MgSO4.7H2O, 0.001% FeSO4.7H2O and 1% carboxymethylcellulose (CMC), after 120 hours of cultivation at 37°C, agitated at 200 rpm. The optimum temperature and initial pH for the endoglucanase production by the A. niger VTCC-F021 strain was 37°C and pH 5. Among tested carbon sources (coffee shell, corncob, dried tangerine skin, glucose, lactose, peanut shell, rice bran, sucrose, and sugar-cane bagasse), yeast extract and sugar-cane bagasse at the concentration of 0.1% (w/v) showed the highest glucanase production with an activity of 6.88 U/ml (100%) and 6.62 U/ml (96%), respectively. Peptone and soybean powder were the best among tested nitrogen sources (NH ) NO , (NH ) SO , fish powder, peptone, soybean powder, urea) with glucanase activity of 5.32 U/ml (100%) and 5.11 U/ml (96%), respectively. A. niger VTCC-F021 produced the glucanase production optimum in the optimal CPY medium containing 0.4% (w/v) of sugar-cane bagasse as the carbon source and 1.4% (w/v) of soybean powder as the nitrogen source