Processed Allogenic Dentine as A Scaffold for Bone Healing: An in vivo study.

Processed Allogenic Dentine as A Scaffold for Bone Healing: An in vivo study Purpose: (i) to assess the osteo-compatibility of dentine in vivo; (ii) to evaluate the ability of liquid nitrogen treated allogenic dentine to accelerate bone healing compared to normal healing in ungrafted defect, and (iii) to compare quantitatively the amount of new bone formation in the allogenic dentine treated defect to autogenous bone treated defect. Methods: Allogenic dentine of New Zealand White rabbits was treated with liquid nitrogen for 2 weeks. In sixteen rabbits, a defect (diameter: 5 mm) was created in each femur for grafting with either allogenic dentine (experimental groups) or autogenous bone (positive control), and in another four rabbits a defect (diameter: 5 mm) was created in each femur and left ungrafted (negative control). The rabbits were sacrificed at 2, 4, 8 and 12-week intervals. Results: Histologically, dentine-bone union has been achieved with no signs of inflammation. Histomorphometric, there was no significant difference in bone regeneration between the groups (£>>0.05). However, the difference was clinically significant between the experimental and negative control groups at 2, 4 and 12 weeks. Conclusion: Results suggest that the liquid nitrogen-treated dentine is osteo-compatible and effective as a bone substitute for accelerating bone repair