Studies on Effect of Pcr-rapd Conditions for Molecular Analysis in Asparagus (Satawari) and Aloe Vera” Medicinal Plants

Molecular marker based analysis of plants relies on high yields of pure DNA samples. This article deals with optimization of DNA isolation and PCR conditions for RAPD analysis of medicinal plants, Asparagus and Aloe vera. The method involves a modified CTAB extraction including addition of PVP, .3M Nacl along with CTAB. The DNA isolated was used for randomly amplified polymorphic DNA (RAPD) analysis. RAPD protocol was optimized using different concentrations of Mg cl 2, Taq polymerase, genomic DNA, primer annealing temperature. Reproducible amplifiable products were observed in PCR reactions.