Author/Authors :
Nanda، Mahoor نويسنده Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi , , Sameer، A. Syed نويسنده Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir , , Syeed، Nidda نويسنده Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir , , Shah، Zaffar A نويسنده Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir , , Murtaza، Imtiyaz نويسنده Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi , , Siddiqi، Mushtaq نويسنده Department of Immunology and Molecular Medicine, Sher-I-Kashmir Institute of Medical Sciences, Soura, Srinagar, Kashmir , , Ali، Arif نويسنده Department of Biosciences, Jamia Millia Islamia, Jamia Nagar, New Delhi ,
Abstract :
Purpose: To assess the frequency of specific point mutations in the K-ras gene in a group of Kashmiri patients with bladder cancer. Materials and Methods: We analyzed the incidence of K-ras exon 1 gene mutations in tumors and surgical margins in 60 patients with transitional cell carcinoma of varied clinical stages and histological grades using the polymerase chain reaction-single strand conformation polymorphism and DNA sequencing. Results: A significant correlation was found between the K-ras, the lymph node status, and tumor recurrence (P < 0.05). Also, smokers and patients with higher tumor grade showed a significantly higher relative risk of developing K-ras mutations than the normal ones. Conclusion: K-ras exon 1 gene mutations were found with low frequency in the bladder cancer tumors from Kashmir valley, which suggests that K-ras gene might be involved in a sub-set of bladder tumors, but it needs further investigation on a larger cohort sample to authenticate the current findings.
