Indirect Organogenesis is Useful for Propagation of Iranian Edible Wild Asparagus (Asparagus officinalis L.)

In order to establish an efficient in vitro propagation protocol for Iranian edible wild Asparagus, this experiment was carried out. For this purpose, explants were excised from spear lateral buds and cultured on MS medium containing 6% sucrose and different NAA and BAP concentrations for both callus induction and shoot development. The treatment inducing the formation of the highest number of shoots was achieved on a medium containing 0.015 mg/l NAA and 0.5 mg/l BAP. For root formation, media were supplemented with two IBA concentrations (1.25 and 1.5 mg/l), that 1.5 mg/l giving a higher rooting percentage (43%). These preliminary results showed that the treatment containing 0.015 mg/l NAA, 0.5 mg/l BAP and 1.5 mg/l IBA gave the highest number of plantlets. Also, The data present a relatively efficient method for in vitro culture of this valuable vegetable in Persia