Identification of RAPD Marker for Chromosome 1D of Common Wheat

Development of genetically compensating nullisomic-tetrasomic and ditelosomic lines of common wheat (Triticum aestivum L.) have been widely used to construct high density genetic maps of homoeologous wheat chromosomes. During present research, easier, cheaper and quicker procedure of Polymerase Chain Reaction (PCR) was used to map Randomly Amplified Polymorphic DNA primers on chromosome 1D of common wheat. Genomic DNA was isolated from two genetic stocks of wheat cultivar Chinese Spring viz; NT-1D1B and NT-2A2B. PCR were conducted using RAPD primers GLC-07 and GLC-11. RAPD primer GLC-11 amplified a polymorphic allele of approximately 500 bp, which was present in NT-2A2B (used as positive control) but was absent in NT-1D1B indicating that the locus is present on chromosome 1D of common wheat. Hence this marker (GLC-11) can reliably be used to keep track of chromosome 1D of hexaploid wheat.