Study of total seed storage protein in wheat varieties based on sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE)

Wheat (Triticum aestivum L.) seed-storage proteins represent an important source of food and energy.Wheat grains of thirteen varieties were collected from different ecological regions of iran. The variability of seed storage-proteins was analyzed by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Electrophorogram for each variety were scored and presence or absence of each band noted and was entered in a binary data matrix. Based on the data of SDS-PAGE gels cluster analysis was performed to check the variations among varieties. Jaccard„s similarity index (JSI) was calculated. Genetic diversity of wheat was evaluated by constructing the dendrogram for high molecular weight (HMW) and low molecular weight (LMW) gluten subunit bands. It is concluded that SDS-PAGE analysis of wheat endosperm protein is useful for evaluation of genetic variability and cultivars identification that help in wheat breeding program.