Author/Authors :
Castelo-Baz، Pablo نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela , , Mart?n-Biedma، Benjam?n نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela , , Ru?z-Pi??n، Manuel نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela , , Rivas-Mundi?a، Berta نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela , , Bahillo، Jose نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela , , Perez-Estévez، Antonio نويسنده Unit of Microbiology, School of Medicine and Dentistry, University of Santiago de Compostela , , Gude، Francisco نويسنده Department of Clinic Epidemiology. University of Santiago de Compostela. Complejo Hospitalario Universitario De Santiago de Compostela, Choupana , , De Moor، Roeland نويسنده Department of Operative Dentistry and Endodontology, School of Dentistry, Ghent University, Ghent University Hospital , , Varela-Pati?o، Purificaci?n نويسنده Unit of Dental Pathology and Therapeutics II, School of Medicine and Dentistry, University of Santiago de Compostela ,
Abstract :
Background: Laser irradiation has been introduced in endodontic treatment due to its bactericidal effect. The aim of this study is to evaluate the bactericidal efficacy of a 940 nm diode laser alone or in combination with 5% sodium hypochlorite (NaOCl) against mature biofilms of E. Faecalis.
Methods: Sixty-eight (60 for the three groups, 4 for SEM and 4 as negative controls) single-rooted human central incisors were prepared and contaminated with E. Faecalis. After two weeks of incubation, specimens were randomly divided in three groups; group 1 (n =20), the teeth were irradiated with a 940 nm diode laser; group 2 (n=20), specimens were rinsed with 5% NaOCl; group 3 (n=20), the teeth were rinsed with 5% NaOCl and then were irradiated with 940 nm diode laser. Four teeth were used to observe the biofilms by SEM. Intracanal bacteria sampling was done, and the samples were plated to determinate the CFU count.
Results: At 24 hours and 7 days, group 3 showed a significant difference (P=0,02; P=0,00) in disinfection if compared to group 1 but did not show this difference if compared to group 2 (P=1, P=0,66), although group 3 obtaining a more extensive disinfection. Groups 1 and 2 did not show difference after 24 hours (P=0,09) but showed a significant difference 7 days afterwards (P=0,04).
Conclusion: The combination of sodium hypochlorite and diode laser light (940 nm) has a synergistic effect, intensifying the bactericidal action.
