Title of article :
Evaluation of Two Immunodiagnostic Assays (MAT and IFA) for Human Leptospirosis in Gilan Province - Iran
Author/Authors :
Mehrabian، Sedighe نويسنده Department of Microbiology, Faculty of Biosciences, Islamic Azad University, Tehran North Branch, Tehran , , Khandan Dezfully، Nooshin نويسنده Department of Microbiology, Faculty of Biosciences, Islamic Azad University, Tehran North Branch, Tehran ,
Issue Information :
فصلنامه با شماره پیاپی 18 سال 2012
Abstract :
Background: Leptospirosis is a systemic infectious disease caused by pathogenic bacteria
belonging to the species of Leptospira, it is considered to be the most common zoonosis
in the world. This is a serious and sometimes fatal infection which is transmitted
from animals to humans by entering the digestive tract or mucosal membrane. Within
the initial 10-12 day period, the patient may experience no or relatively minor symptoms,
then the bacteria enters the blood system and causes contamination of the internal organs,
especially the liver.
Objectives: This study prospectively evaluated two serological methods, the microscopic agglutination
test (MAT) and indirect immunofluorescence assay (IFA) with the aim of identifying
leptospiral agents quickly, in order to prevent and control leptospirosis diseases.
Patients and Methods: In this study, 90 blood samples and their serums, were collected
from patients showing clinical symptoms of leptospirosis, and these were analyzed with
MAT and IFA tests, to test for the presence of leptospiral agglutinins. In this study we
used five serogroups of Leptospira interrogans, which have been identified as the dominant
serogroups in Iran (Pomona, Canicola, Grippotyphosa, Icterohaemorrhagiae and
Sejro hardjo).
Results: The results obtained showed that when compared to the IFA, the MAT was a
more sensitive test. The IFA method also identified certain specific properties of the serogroups;
Pomona, Canicola and Icterohaemorragiae, which are detailed in the article.
Conclusions: Serological testing is the most widely used method for diagnosing leptospirosis,
and the MAT is considered to be the gold standard test for the diagnosis and
detection of leptospiral antibodies in both human and animal sera, for the infecting serovar
and serogroup.
Journal title :
Jundishapur Journal of Microbiology (JJM)
Journal title :
Jundishapur Journal of Microbiology (JJM)