Assessment of Antibacterial Capability of Rhamnolipids Produced by Two Indigenous Pseudomonas aeruginosa Strains

In recent decades, biological properties of biosurfactants, particularly glycolipids, including antimicrobial, antifungal, and anti-cellular efects have been pro- jected in many research studies. The unique advantages of these compounds in terms of production and environment made them important as antimicrobial agents. Objectives: This study aims to evaluate probable antibacterial and antifungal proper- ties of MR01 and MASH1 biosurfactants against several specifed microorganisms. Materials and Methods: In this article antibacterial activities of two rhamnolipid mixtures of MR01 and MASH1 were studied. MR01 and MASH1 are glycolipid-type biosur- factants that are being produced by two strains of Pseudomonas aeruginosa MR01 and MASH1, respectively. Antibacterial efects of two biosurfactants were assessed by disc difusion test method and determination of minimum inhibitory concentration (MIC). Results: They exhibit not only excellent surface activity but also remarkable inhibitory efect against Gram-positive bacteria. According to results, although none of two biosur- factants showed signifcant efects on Gram negative bacteria growth inhibition, both assessing methods confrmed the growth inhibition of Gram-positive bacteria by MR01 and MASH1 biosurfactants. Conclusions: According to results of this study, MR01 and MASH1 biosurfactants had antimicrobial efcacy against Gram positive bacterial groups. This efect is comparable to antibiotics and therefore the future use of these biosurfactants as broad spectrum antibiotics is highly promising. These features of biosurfactants (BS) should broaden its applications in new advanced technologies. Future studies will be performed on characterization and isolation of biologically active fraction of the rhamnolipid biosurfactant produced by P. aeruginosa strains. This bioac- tive compound may be evaluated as a potent antimicrobial agent to be applied against a panel of pathogenic micro-organisms including a few multidrug-resistant (MDR) clini- cal pathogenic isolates such as methicillin-resistant Staphylococcus aureus (MRSA) and other MDR pathogenic strains .