Cross Reactivity between Dromedary Whey Proteins and IgG Anti Bovine a-Lactalbumin and Anti Bovine b-Lactoglobulin

Problem statement: Our aim was to enhance the data on antigenic properties of dromedary whey proteins. Approach: The identification of the whey proteins was carried by SDS-page and Reversed phase high performance liquid chromatography (RP-HPLC). The cross-reactivity of dromedary whey proteins with IgG anti bovine b-lactoglobulin and anti bovine a-lactalbumin, obtained by immunisation of Balb/c mice, was carried out by ELISA. Results: The SDS-page showed the presence of band corresponding to a-lactalbumin and albumin serum; this was confirmed by the chromatogram obtained by RP-HPLC, where we detected a pick corresponding to a-lactalbumin. There was a cross reaction of dromedary whey proteins with IgG anti bovin a-lactalbumin but it was very weak with IgG anti boin b-lactoglobulin. Conclusion: We detected a cross reactivity between dromedary whey proteins and IgG anti bovin a-lactalbumin.