Molecular and cytological evaluation of male sterile and restorer lines in hybrids rice

ABSTRACT: Information on the genetics of fertility restoration facilitates breeding and/or selection of restorer lines used in hybrid breeding program in a cytoplasmic male sterility (CMS) system. Cytoplasmic male sterility (CMS) is a common phenomenon that has been extensively used for production of hybrid seeds in various crops. Rf genes are needed for restoring fertility to CMS lines. Searching for molecular tagging of restorer genes is of high importance where phenotyping is very time consuming and requires the determination of spikelet sterility in testcross progeny. In order to determine maintainers and fertility restorers lines in hybrids rice, 5 cytoplasmic male sterile was crossed whit 10 pure line as tester. In other year, F1 progenies arranged in RCBD design whit three Replications. Evaluation of fertility in F1 populations showed IR-9 parent for all the crosses was identified as restorer. The cross Rome parent with CMS lines observed lowest amount fertility, also molecular analysis confirmed exist gene RF only in IR-9 parent.