Author/Authors :
Carata ، Elisabetta نويسنده Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy Carata , Elisabetta , Tenuzzo ، Bernardetta Anna نويسنده Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy Tenuzzo , Bernardetta Anna , Arno، Federica نويسنده Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy Arno, Federica , Buccolieri ، Alessandro نويسنده Department of Materials Science, University of Salento, Lecce, Italy Buccolieri , Alessandro , Serra ، Antonio نويسنده Department of Materials Science, University of Salento, Lecce, Italy Serra , Antonio , Manno ، Daniela نويسنده Department of Materials Science, University of Salento, Lecce, Italy Manno , Daniela , Dini ، Luciana نويسنده Department of Biological and Environmental Sciences and Technologies, University of Salento, Lecce, Italy Dini , Luciana
Abstract :
Members of the 14-3-3 protein family are involved in many important cellular events, including stress response, survival and apoptosis. Genes of the 14-3-3 family are conserved from plants to humans, and some members are responsive to UV radiation. Despite the high rate of pollution generated by nano-pollutants, up to now their toxic effect on development is totally obscure. Embryos treated with carbon nanoparticles, RNA preparation, retro-transcription and quantitative real-time PCR. In response to carbon nano-particles exposure, the embryos collected 24 h later showed a 3,07-fold at 5x1012 p and a 1,58-fold at 2.5x1013 p and a 1,92-fold at 2.5x1014 p increase in Pl14-3-3? transcript levels compared with controls. The Pl14-3-3? mRNA delocalization parallels the failure in archenteron elongation observed morphologically, as well as the lack of specific endoderm markers. Here, we report the isolation of the complete cDNA encoding the 14-3-3 epsilon isoform from Paracentrotus lividus sea urchin embryos, referred to as Pl14-3-3?. Pl14-3-3? mRNA levels were measured by RT-PCR during development and found to increase from the mesenchyme blastula to the prism stage. Our results confirm the involvement of 14-3-3? in the stress response elicited by carbon nano-particles.
