Author/Authors :
I، Sainova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , I، Vavrek نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , V، Pavlova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , T، Daneva نويسنده Department of Immunobiology of Reproduction, Institute of Biology and Immunology of Reproduction to Bulgarian Academy of Sciences, 1113 Sofia, , , I، Iliev نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , L، Yosifova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , E، Gardeva نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, , , E، Nikolova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences, ,
Abstract :
Taking in consideration the importance of coordinated oncogenes and tumor-suppressor genes activity in the control of malignancies and death, additional copy of oncogene Dcn1 was inserted in mouse embryonic stem cells (mESCs) by transfection with recombinant DNA-constructs. The noticed absence of phagocyte differentiation of myeloid progenitors, co-cultivated with the so derived mESCs, differed from the data, observed in co-cultivation with malignant cells, containing and non-containing additional copy of tumor-suppressor gene. These data were a proof for preserved non-malignant cell characteristics of the transfected mESCs and were confirmed by their analogy with normal human trophoblasts HTR8/SVneo, immortalized by transfection with recombinant vector SV40. On the other hand, the analogy of malignant rat insulinoma cells RIN-5F with human cervical carcinoma cells HeLa, was also similarly tested. In this connection, the levels of phagocyte differentiation of myeloid progenitors in their co-cultivation with RIN-5F cells, containing additional copy of tumor-suppressor gene SCGN, as well as with HeLa cells with additional copy of tumor-suppressor gene HACE1, were differed of the observed in their co-cultivation with HeLa cells, non-containing additional tumor-suppressor gene. Analogus functions of protein products of both genes SCGN and HACE1 have been indicated as tumor suppressors by their participation in similar cascade mechanisms, such as interactions with cytoskeleton elements, interacting with nuclear proteins. Future studies are necessary in that direction.
