Author/Authors :
I، Sainova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , I، Vavrek نويسنده 1Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , V، Pavlova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , T، Daneva نويسنده Department of Immunobiology of Reproduction, Institute of Biology and Immunology of Reproduction to Bulgarian Academy of Sciences, 1113 Sofia, Bulgari , , I، Iliev نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , L، Yossifova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , E، Gardeva نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences , , E، Nikolova نويسنده Department of Experimental Morphology, Institute of Experimental Morphology, Pathology and Anthropology with Museum to Bulgarian Academy of Sciences ,
Abstract :
Taking in consideration many literature data, according which coordinated oncogenes and tumor-suppressor genes functions is of most importance. In this connection, the main idea was balanced activity of oncogenes and tumor-suppressor genes in vitro in laboratory-cultivated normal cells be achieved. Additional copy of oncogene was inserted Dcn1 in mouse embryonic stem cells (mESCs) by their transfection with appropriate recombinant DNA-constructs. On the other hand, in cultivation of separate sub-populations of non-transfected mESCs in the presence of Doxicyclin, suppressed cell proliferation and increased myeloid cell differentiation was observed, probably by activation of genes from STAT-family. After in vitro-co-cultivation of sub-populations of transfected mESCs, containing additionally-inserted copy of oncogene Dcn1 with sub-populations of non-transfected mESCs, treated with Doxicyclin, the results obtained have indicated the safety and preserved normal/non-tumorigenic cell character of the so derived stem cells in vitro, despite of the presence of additional oncogene copy. These data were confirmed by results from similar experiments with analogical human normal trophoblast cells, immortalized by transfection with SV40 recombinant gene vector.
