Author/Authors :
Cevik، Emre نويسنده Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey Fatih University, Department of , , ?enel، Mehmet نويسنده Department of Chemistry, Faculty of Arts and Sciences, Fatih University, B.Cekmece, Istanbul 34500, Turkey , , Abas?yan?k، M. Fatih نويسنده Department of Genetics and Bioengineering, Faculty of Engineering, Fatih University, B.Cekmece, Istanbul 34500,Turkey Fatih University, Department of ,
Abstract :
A novel amperometric hydrogen peroxide biosensor was fabricated for the
determination of H2O2. Catalase (Cat) was immobilized on a glassy carbon electrode by
poly(glycidyl methacrylate-co-vinylferrocene) (poly(GMA-co-VFc)). A polymeric electron
transfer mediator, containing copolymers of glycidyl methacrylate (GMA) and
vinylferrocene (VFc) with different molar ratios, have been prepared by free radical
copolymerization. Amperometric response was measured as a function of concentration of
H2O2, at fixed potential of +0.35 V vs. Ag/AgCl in a phosphate buffered saline (pH 7.5). The
mediated hydrogen peroxide biosensor had a fast response of less than 7 s with linear range
0.5-14 mM. The sensitivity of the biosensor for H2O2 was 34 nA/mM.cm2.
