Author/Authors :
Mousavi، E. S. نويسنده Department of Horticulture, Karaj Branch Islamic Azad University, Karaj, Iran , , Hadavi، E. نويسنده Department of Horticulture, Karaj Branch Islamic Azad University, Karaj, Iran , , Behbahani، S. M. R. نويسنده , , Miri، S. M. نويسنده Department of Horticulture, Karaj Branch Islamic Azad University, Karaj, Iran ,
Abstract :
This study was conducted to evaluate the effect adding hormones on callus induction and in vitro
regeneration from callus of Lisianthus (Eustoma grandiflorum) by using a completely randomized
design and factorial experiment. Sterile explants were placed on basal medium B5 along with different
concentration of 2, 4-D and NAA under dark and light conditions. In regard to the explants location
collogenesis was happened just from leaf explants. Increasing the amount of NAA, in referring the
hormonal balance, lead to more collogenesis than 2, 4-D (P < 0.05). For indirect regeneration callus was
subcultured in B5 medium supplemented with different concentration of Kin or BAP which applied
individually or in combination with NNA, IAA and GA3. Media contains GA3 and Kin induced
maximum proliferation from callus.
