EFFICIENCY OF SEPTORIA TRITICI SPORULATION ON DIFFERENT CULTURE MEDIA

The solid and liquid media were investigated for their efficiency in quantitative sporulation of Septoria tritici. The inoculated culture media were incubated at 18 to 20 °C with a photoperiod of 12 h light and 12 h darkness and investigated after 4 to 6 days. Among solid media, YMDA (yeast extract + malt extract + dextrose + agar) and liquid media YMB (yeast extract + malt extract + glucose in 1 lit distilled water) were the best with conidia production rates of 1.7 × 109 spore/ ml and 2.3 × 109 spore/ ml, respectively. The pathogenicity tests using conidia produced on PDA, YMDA and YMB showed that the inoculum produced on YMDA and YMB are more successful in establishing disease in wheat than inoculum produced on PDA. These results indicate that YMDA and YMB are the most efficient culture media inoculum production and the most reliable inoculum production method for screening of wheat cultivars for resistance to Septoria leaf blotch.