Author/Authors :
Salehi، Mohammad Saied نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Jafarzadeh Shirazi، Mohammad Reza نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Javad Zamiri، Mohammad نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Pazhoohi، Farid نويسنده Department of Animal Science, College of Agriculture, Shiraz University, Shiraz, Iran , , Namavar، Mohammad Reza نويسنده Histomorphometry and Stereology Research Center , Department of Anatomical Sciences, School of Medicine, Shiraz University of Medical Sciences, Shiraz , , Niazi، Ali نويسنده , , Ramezani، Amin نويسنده Department of Medical Biotechnology, School of Advanced Medical Sciences and Technologies, Shiraz University of Medical Sciences, Shiraz, Iran , , Tanideh، Nader نويسنده , , Tamadon، Amin نويسنده Department of Clinical Sciences, School of Veterinary Medicine, Shiraz University & Stem Cell and Transgenic Technology Research Center, Shiraz Uni Tamadon, Amin , Zarei، Afsoon نويسنده Infertility Research Center & IVF Center, Shiraz University of Medical Sciences, Shiraz, Iran ,
Abstract :
Kisspeptin and RFamide-related peptide-3 (RFRP-3) are known to affect GnRH/luteinizing
hormone (LH) in several species, including the rat. It has been hypothesized that GnRH/LH changes
during the rat estrous cycle may result from changes in the expression of KiSS1 and RFRP-3 genes.
Therefore, the present study investigates KiSS1 and RFRP-3 gene expression at the transcriptional
level in the rat hypothalamus during the estrous cycle.
In the present experimental study, 36 adult female Sprague-Dawley rats (3-4 months old) were used to
study the expression of KiSS1 and RFRP-3 mRNA in the hypothalamus during the estrous cycle. Four
rats were ovariectomized, whereas the remainder were allotted to four different phases of the estrous
cycle (n=8 per estrus phase). Rats were decapitated, and the hypothalami were immediately dissected and
frozen in liquid nitrogen. Expressions of KiSS1 and RFRP-3 mRNAs were analyzed by real-time PCR.
The expression of KiSS1 mRNA during estrus was lower than other phases of the cycle (p < 0.01).
Expression of KiSS1 mRNA during the metestrus phase was lower than the proestrus phase (p < 0.01).
The expression of RFRP-3 mRNA during proestrus was lower than the diestrus phase (p < 0.01).
Results of the present study showed the role of coordinated expression of KiSS1 and RFRP-3 mRNA
in the hypothalamus in the control of the rat estrous cycle.
