Author/Authors :
Azadi Gonbad، Reza نويسنده Tea Research Institute of Iran, 44159-77555, Lahijan, Iran. , , Siavash Moghaddam، Sina نويسنده Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300, Serdang, Malaysia , , Sinniah، Uma Rani نويسنده Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300, Serdang, Malaysia , , Abdul Aziz، Maheran نويسنده Department of Agriculture Technology, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300, Serdang, Malaysia , , Safarpor، Mahdi نويسنده Department of Crop Science, Faculty of Agriculture, Universiti Putra Malaysia (UPM), 43300, Serdang, Malaysia ,
Abstract :
Establishment of in vitro plantlets in the greenhouse on various additives of soil mixtures is considered as one of the trickiest phases of micropropagation. In the present research, we report the results of an experiment aimed at optimizing acclimatization of tea clone Iran 100 plantlets (Camellia sinensis (L.) O. Kuntze) by using different types and ratios of potting media. Potting media like peat moss, vermiculite and perlite with different ratios were mixed together. This experiment was done in four repetitions using the randomized complete block design. The properties such as survival rate, number of leaves, plant height and root length were measured and recorded in the period of the experiment. The collected data was analyzed by SAS statistical software and the mean values were evaluated by the Tukey Test using the probability of 1 percent. The mixture containing peatmoss + vermiculite + perlite (2:1:1; v/v/v) resulted in increased percentage of plant survival, root length, plant height and number of leaves of tea clone Iran 100 and it is therefore, a recommended medium for growth of the cultivar during acclimatization.
