Title of article
Resolution of N-linked oligosaccharides in glycoproteins based upon transglycosylation reaction by CE-TOF-MS
Author/Authors
Fukushima، Takeshi نويسنده , , Toyooka، Toshimasa نويسنده , , Kato، Masaru نويسنده , , Min، Jun Zhe نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
-3483
From page
3484
To page
0
Abstract
The resolution of asparagine-type oligosaccharides in glycoproteins was carried out by combination of the transglycosylation reaction and CE-TOF-MS. The oligosaccharides enzymatically transferred to a fluorescent acceptor (NDA-Asn-GlcNAc) with Endo-M. The resulting fluorescent-oligosaccharides were separated by CE and detected by TOF-MS. Disialo-Asn was successfully identified by the proposed procedure. Application to oligosaccharides in ovalbumin was also described in this communication.
Keywords
molecular data , molecular processes , ISM: molecules
Journal title
CHEMICAL COMMUNICATIONS - LETCHWORTH
Serial Year
2005
Journal title
CHEMICAL COMMUNICATIONS - LETCHWORTH
Record number
69287
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