Title of article
Stereoselective hydroxylation of an achiral cyclopentanecarboxylic acid derivative using engineered P450s BM-3
Author/Authors
Arnold، Frances H. نويسنده , , Munzer، Dieter F. نويسنده , , Meinhold، Peter نويسنده , , Peters، Matthew W. نويسنده , , Feichtenhofer، Sabine نويسنده , , Griengl، Herfried نويسنده , , Glieder، Anton نويسنده , , Raadt، Anna de نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2005
Pages
-2596
From page
2597
To page
0
Abstract
Substrate engineered, achiral carboxylic acid derivative 2 was biohydroxylated with various mutants of cytochrome P450 BM-3 to give two out of the four possible diastereoisomers in high de and ee. The BM-3 mutants exhibit up to 9200 total turnovers for hydroxylation of the engineered substrate, which without the protecting group is not transformed by this enzyme.
Keywords
Spurgia esulae , Biological control , IPM , Invasive weeds , Endangered species , Aphthona czwalinae , Euphorbia esula , Aphthona lacertosa , Leafy spurge flea beetles , Aphthona flava , Aphthona nigriscutis
Journal title
CHEMICAL COMMUNICATIONS - LETCHWORTH
Serial Year
2005
Journal title
CHEMICAL COMMUNICATIONS - LETCHWORTH
Record number
70017
Link To Document