Targeting of mixed sequence double-stranded DNA using pyrene-functionalized 2-amino(alpha)-L-LNA

Incorporation of a single pyrene-functionalized 2-amino(alpha)-L-LNA monomer X into short DNA strands induces extraordinarily high binding affinity towards complementary DNA (up to 16 °C increase per modification), whereas labile duplexes, suitable as probes for targeting of double stranded DNA, are formed upon positioning of two monomers X in an interstrand +1 zipper motif.