Author/Authors :
Hashemibeni، Batool نويسنده Department of Anatomical Sciences and Molecular Biology, Medical School, Isfahan University of Medical Sciences, Isfahan , , Jafary، Farzaneh نويسنده Department of Biology, Sciences School, Alzahra University, Tehran , , Esmaeil، Nafiseh نويسنده Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan , , Goharian، Vahid نويسنده Department of Surgery, Amine hospital, Isfahan University of Medical Sciences, Isfahan , , Feizi، Ghader نويسنده Torabinegad Research Center, Department of Endodontics, Dental School, Isfahan University of Medical Sciences, Isfahan , , Heidari، Fariba نويسنده Torabinegad Research Center, Department of Endodontics, Dental School, Isfahan University of Medical Sciences, Isfahan , , Moosavi-Nejad، Sayyedeh Zahra نويسنده Department of Biology, Sciences School, Alzahra University, Tehran , , Zarkesh-Esfahani، Sayyed Hamid نويسنده PhD, Department of Immunology, Medical School, Isfahan University of Medical Sciences, Isfahan ,
Abstract :
Background: Characteristics of differentiated osteoblasts from adipose
derived stem cells (ADSCs) in compared with isolated osteoblasts from
normal bone such as calvaria are unknown. The aim of this study was
determination and comparison of phenotypic characterization between
differentiated osteoblasts from stem cells and calvaria osteoblasts in vitro.
Methods: In this study, mesenchymal stem cells were isolated from
adipose tissue of human by enzymatic digestion and were differentiated
into osteoblasts using osteogenic medium. Characteristics of these cells
at first, second, third and fourth weeks were comprised with calvaria
osteoblasts that were isolated from human calvaria by explanation
culture method. To screen the characteristics of both calvaria and the
differentiated osteoblasts, we used western blot to identify protein
levels, von Kossa staining for mineral matrix detection and alkaline
phosphatase (ALP) assay kit (Sigma) for ALP activity measurement.
Difference between calvaria and differentiated osteoblast cells were
analyzed by one?way ANOVA and P < 0.05 was considered as statistically
significant.
Results: Alkaline phosphatase activity, collagen and mineral material
production in differentiated osteoblasts at third week were more
significantly than calvaria cells (P < 0.05). Our results indicated that there
was no significant different in osteocalcin (OC) production between
differentiated osteoblast at first, second and third weeks and calvaria
cells but declined at fourth week (P < 0.05).
Conclusions: Our survey showed that cellular traits of differentiated
osteoblasts presented better than calvaria osteoblasts in vitro conditions.
Therefore, we suggest that ADSCs could be used in next studies for
bone tissue engineering.
