GlutathioneS-Transferase Activity in Aquatic Macrophytes with Emphasis on Habitat Dependence

GlutathioneS-transferase activity of both the microsomal and soluble fractions was determined in a variety of aquatic macrophytes. The examined enzyme extract was prepared from a combination of leaves and shoots. Four different model substrates were used. The highest conjugation rate was obtained for 1-iodo-2,4-dinitrobenzene, followed by 1-chloro-2,4-dinitrobenzene,p-nitrobenzoyl chloride, and 1,2-dichloro-4-nitrobenzene. Comparison of several samples ofNuphar luteaL. from two different lake areas revealed increased glutathioneS-transferase activity in plants from the site contaminated with polyaromatic hydrocarbons.