Photo-induced toxicity of four polycyclic aromatic hydrocarbons, singly and in combination, to the marine diatom Phaeodactylum tricornutum

Polycyclic aromatic hydrocarbons (PAHs) enter the aquatic environment by various routes and are usually found as mixtures in the water. Many studies have shown that solar ultraviolet (UV) radiation can greatly enhance the toxicity of some PAHs to a variety of marine species. In the present study, we tested the phototoxicity of four PAHs with simple structures, both alone and in binary combinations, to a species of marine diatom, Phaeodactylum tricornutum, in the laboratory. The results indicated that simulated solar UV radiation not only enhanced the toxicity of the different PAHs to this alga, but also changed their relative toxic strengths. The photo-induced toxicity of PAHs to this alga might be a synergistic effect of photo-modification and photosensitization reactions, causing the microalgal cells to suffer oxidative stress. Four binary mixtures of these PAHs were found to have a synergistic joint action mode, while two binary mixtures displayed an antagonistic reaction, revealing a complex pattern of possible interactions of PAHs with marine diatoms.