Bioaccumulation and toxic effects of elevated levels of 3,3′,4,4′-tetrachloroazobenzene (33′44′-TCAB) towards aquatic organisms. II: Bioaccumulation and toxic effects of dietary 33′44′-TCAB on the Japanese Medaka (Oryzias Latipes)

The polychlorinated aromatic compound 3,3′,4,4′-tetrachloroazobenzene (33′44′-TCAB) is a contaminant of 3,4-dichloroaniline (3,4-DCA) based herbicides, and of agricultural soils. In this study, the Japanese Medaka (Oryzias latipes) was exposed to 0.5 – 2500 ppm 33′44′-TCAB through its diet. There was a distinct trend towards increased mortality and increased body burdens of 33′44′-TCAB with increasing 33′44′-TCAB charge in the food. At the highest levels of exposure, there was a significant number of excess deaths compared to control groups.