Isomer-specific detection of PAHs and pah metabolites in environmental matrices by Shpolʹskii luminescence spectroscopy

Shpolʹskii Spectroscopy, a high-resolution low temperature molecular luminescence technique, was applied to the analysis of various complex environmental matrices to obtain isomer specific information on a wide range of polycyclic aromatic hydrocarbons (PAHs) and their derivatives. Using conventional lamp excitation and fluorescence and phosphorescence detection, parent priority PAHs, six-membered ring compounds (e. g. potent carcinogens, dibenzopyrenes), and methyl-substituted PAHs were determined in crude and purified lubricating oil sample, extracts of sediment, and muscle from finfish. In addition, detection of major PAH metabolites (hydroxy-substituted PAHs) in gall bladder of fish is demonstrated by applying conventional lamp and Laser Excited Shpolʹskii Spectroscopy (LESS).