Internal lethal concentration and internal lethal volume fraction of chlorobenzenes in mosquito fish (Gambusia affinis)

The internal lethal concentration (ILC) and internal lethal volume fraction (ILVF) of a series of chlorobenzenes with mosquito fish (Gambusia affinis) were measured by a semistatic test approach in closed jars. Their variation with exposure times were also investigated. With different aqueous chemical concentrations and different exposure periods, the measured ILC values on wet weight and lipid weight bases ranged from 3–12 mmol kg−1 and 46–208 mmol kg−1 lipid, respectively. Measured ILVF values covered a range from 0.008-0.025 (L toxicant L−1 lipid). These values are consistent with those found in literature. Generally ILC and ILVF values decreased with increasing exposure time with rate constants ranging from 0.03 to 0.33 day−1 (wet and lipid weight) for ILC and 0.03 to 0.31 day−1 for ILVF.