The influence of quinones and phenols on the triple NAD(H)-dependent enzyme systems

Kinetics of the triple bioluminescent enzyme system:alcohol dehydrogenase - NADH:FMN oxidoreductase - luciferase in the presence of quinones and phenols has been studied. The correspondence between the bioluminescent kinetic parameters, redox potentials and concentrations of the quinones and phenols has been estimated. The substances have been shown to change bioluminescent kinetics through moving off the NAD+/NADH balance in the enzyme processes. This system is proposed to be used as enzymatic biotest in ecological monitoring.