Development of an optimal bacterial medium based on the growth inhibition assay with Vibrio fischeri

Chronic toxicity level of chemicals to bacteria can depend on composition and concentration of medium ingredients. This was demonstrated by means of a growth rate inhibition test with the marine bacterium V. fischeri. In a minimal medium (following the validity criterion of achieving a least cell multiplication rate) containing only yeast extract as organic nutrient component, V. fischeri was to Cu2+ 11,9 times, to Hg2+ 3 times and to Zn2+ 2,8 times more sensitive than in the complete defined medium. Obviously yeast extract did not interfere greatly in the complexation mechanisms. The detection limits of the toxicity of Cd2+ 3,5-dichorophenol and nitrobenzene were not influenced by the substrate.