Biochemical and Histopathological Changes in Serum Creatinine and Kidney Induced by Inhalation of Thimet (Phorate) in Male Swiss Albino Mouse, Mus musculus

This work was conducted to investigate the biochemical and histopathological changes in serum creatinine level and kidney of male Swiss albino mouse, Mus musculus, exposed to the recommended field dose of Thimet (20 kg ha−1). The animals were exposed to this dose in a whole-body inhalation chamber for 12 weeks and the biochemical and histopathological changes were studied in the 2nd, 4th, 6th, 8th, 10th, and 12th weeks of exposure. Creatinine level of serum was measured by the Jaffe method, and histopathological lesions were studied by the hematoxylin–eosin staining method. A significant rise in creatinine level was observed from the 4th week of exposure until the end of the experiment. This rise suggests impairment of the glomerular function and tubular damage in the kidneys. These changes were confirmed by histopathological studies in the tubules. Kidney lesions were present throughout the experimental period. These consisted of mild to severe multifocal cloudy and hydropic degeneration with necrosis in the tubules, though the glomerular damage was not seen by light microscopy. After a 30-day recovery period, the histopathological and biochemical changes were absent and normal patterns were restored.