Graphical estimation of the dual-enzyme kinetic parameters for Cr(VI) reduction

Chromium(VI) (Cr(VI)) contamination of soil and groundwater is a major environmental concern. Bioreduction of Cr(VI) by Shewanella oneidensis MR-1 (MR-1) can be considered a feasible option to reduce the toxic and mobile Cr(VI) to the less toxic and less mobile chromium(III) (Cr(III)). The reaction rate expression for Cr(VI) reduction is nonlinear and the rate constants are evaluated by employing nonlinear optimization techniques. The outcome of the optimization techniques, in general, depends on the initial estimate of the kinetic parameters which is not always available. A graphical approach based on sound mathematical reasoning has been developed which is accurate, simpler to use, and can provide the best initial estimate for nonlinear optimization.