The influence of food quality on the nutritional acclimation of the copepod Acartia clausi

The influence of food quality on the nutritional metabolism of Acartia clausi was studied experimentally using four different diets: (1) diatom cells of Thalassiosira weissflogii, (2) detritus prepared from the same culture, (3) 50 : 50 mix on a protein basis of the two previous diets, and 4) dinoflagellate cells of Prorocentrum micans. For each trophic, ingestion, gut transit time, trypsin activity and Km (half saturation constant) were measured at limiting and saturating concentration. Assimilation rates were also estimated for both pure diatoms and mixed live–detrital cell diets. Ingestion followed a Holling type 2 response for diets 1 and 4, a linear one for detritus and an intermediate response for diet 3. Gut transit time displayed different adaptive changes with food regime depending on protein concentration. Trypsin activity was lower for detrital food and trypsin Km significantly decreased with increasing concentration of live diatoms. Assimilation rates were higher for live food than for mixed live–detrital food. Results illustrated that Acartia-type copepods optimize nitrogen or protein uptake. They suggest that besides chemoreception-mediated selectivity, internal controls by digestion and assimilation also regulate ingestion (feed-back).