Title of article :
An improved method for the isolation of artifact-free algaenans from microalgae
Author/Authors :
B. Allard، نويسنده , , J. Templier، نويسنده , , C. Largeau، نويسنده ,
Issue Information :
روزنامه با شماره پیاپی سال 1998
Pages :
6
From page :
543
To page :
548
Abstract :
A new process for the isolation of algaenans (insoluble non-hydrolysable, highly aliphatic biopolymers of microalga cell walls) which avoids their contamination with melanoidin-like polymer artifacts is presented and the algaenans of two species from the Chlorophyceae (Scenedesmus communis and Botryococcus braunii) are re-examined. Preliminary release of the polysaccharides from these microorganisms by trifluoroacetic acid hydrolysis affords a pure, or much less contaminated, algaenan for the two species. Re-examination of the pyrolysate of S. communis algaenan indicates that the long-chain n-alkylnitriles, previously detected when the algaenan was isolated by the usual process (Berkaloff et al., 1983), do not actually originate from this biopolymer. We suggest that some nitrogen-containing molecules present in S. communis (or in other Chlorophyceae species) are incorporated into the structure of the melanoidin-like polymers generated when the algaenan is isolated by the usual process which lead, upon pyrolysis, to nitriles. Consideration of previous data, on the pyrolysates of numerous kerogens, suggests that a similar process occurs under natural conditions during early diagenesis.
Keywords :
Green microalgae , insoluble non-hydrolysable biopolymer (INH biopolymer) , algaenan , Scenedesmus communis , Botryococcus braunii , Chlorophyceae , melanoidin-like polymer
Journal title :
Organic Geochemistry
Serial Year :
1998
Journal title :
Organic Geochemistry
Record number :
752501
Link To Document :
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