Oxygen diffusion in active layer of aerobic granule with step change in surrounding oxygen levels

High biomass density and large size limit the transfer of dissolved oxygen (DO) in aerobic granules. In the literature, the oxygen diffusivity is often employed as an input parameter for modeling transport processes in aerobic granules. The interior of an aerobic granule was observed to be highly heterogeneous. In this work, the distributions of extracellular polymeric substances (EPS) and cells in the interior of phenol-fed and acetate-fed granules were built up using a five-fold staining scheme, combined with the use of a confocal laser scanning microscope (CLSM). The steady-state and transient DO with step changes in surrounding DO levels at various depths were measured in the granules using microelectrodes. Cells were probed in a surface layer of thickness 125–375 μm. A marked fall in DO was also noted over this surface layer. No aerobic oxidation could occur beneath the active layer, indicating the oxygen transfer limit. Fitting the steady-state and transient DO data over the active surface layer yielded apparent diffusivities of oxygen were (9.5±3.5)×10−10 m2 s−1 for the phenol-fed granule and (3.5±1.0)×10−10 m2 s−1 for the phenol-fed granule. These values were lower than those adopted in models in the literature.