A comparison of sample handling and analytical methods for determination of acid volatile sulfides in sediment

Proper sample storage and analytical techniques are required when analyzing anoxic sediment for acid volatile sulfide (AVS), because a change in redox state may alter sediment parameters. The goal of this study was to determine the optimum sample handling, storage, and analytical techniques to adopt when determining AVS concentrations in sediment. To determine the correct handling and storage protocols for use with anoxic sediment, samples of three different AVS levels were manipulated under a combination of the following handling and storage plans: handled on benchtop or under nitrogen atmosphere; preserved with zinc acetate (ZnAc) or not preserved, and stored at 4 °C or frozen (< − 20 °C). The data indicate that sulfide levels are best maintained when samples are handled under a nitrogen atmosphere, stored at 4 °C or frozen, and analyzed within 2 weeks of collection. To determine the best analytical procedure for the determination of AVS in sediment, the following methods were compared: colorimetric, gravimetric, ion-selective electrode, and photoionization detection. The colorimetric method was preferred.