Title of article
Characterization of Leishmania sp. strains isolated from autochthonous cases of human cutaneous leishmaniasis in Santa Catarina State, southern Brazil
Author/Authors
Edmundo C. Grisard، نويسنده , , M?rio Steindel، نويسنده , , Jeffrey J. Shaw، نويسنده , , Edna A. Y. Ishikawa، نويسنده , , Carlos J. Carvalho-Pinto، نويسنده , , Iriane Eger-Mangrich، نويسنده , , Helena K. Toma، نويسنده , , José H. Lima، نويسنده , , Alvaro J. Romanha، نويسنده , , David A. Campbell، نويسنده ,
Issue Information
روزنامه با شماره پیاپی سال 2000
Pages
5
From page
89
To page
93
Abstract
Four Leishmania sp. samples were isolated from autochthonous human cases of American cutaneous leishmaniasis (ACL) in Santa Catarina State, southern Brazil. These strains were characterized using indirect immunofluorescence with a panel of Leishmania-specific monoclonal antibodies (MAbs), and by PCR amplification and hybridization assay of the mini-exon gene with group specific probes. The results obtained with the MAbs were in agreement with the genetic marker. Two isolates (MHOM/BR/89/JSC89-H1 and MHOM/BR/89/JSC89-H2) were identified as L. (Leishmania) amazonensis and two (MHOM/BR/96/LSC96-H3 and MHOM/BR/97/LSC97-H4) as L. (Viannia) braziliensis. The southernmost autochthonous cases of ACL in Brazil are due to two different Leishmania sp. species, confirming the spreading of ACL on the American continent
Keywords
Leishmania sp. , monoclonal antibodies , characterization , Human cutaneous leishmaniasis , Hybridization , Spliced leader , PCR , Mini-exon gene
Journal title
Acta Tropica
Serial Year
2000
Journal title
Acta Tropica
Record number
777501
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