Vector competence for Onchocerca volvulus in the Simulium (Notolepria) exiguum complex: Cytoforms or density-dependence?

Although Simulium exiguum Roubaud s.l. is present in all South American onchocerciasis foci, it is a significant vector only in Colombia and Ecuador. This variable vectorial role has been attributed to sibling forms that differ in their ability to allow Onchocerca volvulus larval development and their preferred bloodmeal hosts. Here we evaluate the relationship between parasite availability in human skin and infective larval output measured as (a) number of L3 larvae and (b) proportion of surviving flies with L3s in the Cayapa form of S. exiguum s.l. from Ecuador, taking into account the variation in counts of microfilariae (mf) from 6 skin snips/patient. Comparisons with other cytoforms (Aguarico, Bucay and Quevedo, absent in the main Ecuadorean onchocerciasis foci) are made to suggest the relative roles of intrinsic susceptibility or co-adaptation versus density-dependent parasite uptake. A nonlinear (limitation) relationship, characterised by an initial rapid increase in infective larvae with increasing mf skin density was confirmed for the Cayapa cytoform. The proportion of infective Cayapa flies increased and saturated rapidly (reaching 80% for ≥ 20 mf/mg skin). After adjusting for density dependence, non-Cayapa cytoforms exhibited significantly lower L3 loads and proportions of infective flies for a given mf skin density than Cayapa flies, indicating that the susceptibility of those cytoforms is intrinsically lower than that of the Cayapa cytoform and that the differences observed are not due to density-dependent effects.