Determining morphological behaviors and geneticvariety of rose aphids using RAPD and RFLP-PCR

ABSTRACT : Rose is one of the most beautiful attractive flowers of world which is important in landscaping because of its unique botanical specifics. One of the most important pests of this plant is aphid. In this study, 135 aphid samples were collected from various regions of Isfahan landscapes. Morphological traits and mitochondrial gene sequencing were used for identifying them. According to morphological traits, these samples were belonging to Macrosiphum rosae,aphis gossipy, and metopolophium dirhodum species which diversity in their morphological traits was obvious. Studying the genetic diversity of 16 selected samples was done by RAPD-PCR molecular marker using three primers and RFLP-PCR using restriction enzyme RsaI. Results showed high genetic diversity in studying population. Samples grouping were done better by RFLP marker than RAPD marker. So, all samples which were located in three groups by this method had also high relations in morphological traits. On the other hand, genetic differences were shown better by RAPD for insects of a group which didn’t have similar morphological traits. Then, this method can be used to observe the highest diversity level of population.