Purification of pig and rat liver squalene epoxidase by affinity chromatography

Three novel affinity columns were evaluated for purification of rat and pig squalene epoxidase (SE). Pig SE was adsorbed to a resin bearing an N-cyclopropyl-N-trisnorsqualenamine (TNS-CPA) type functionality, and eluted with 25 mM KCl to give a 55–56 kDa doublet, whereas rat SE required the use of N-methyl-N-trisnor-squaleneamine (TNS-MA) affinity resin. Rat SE could be eluted with 0.5M KCl, to give a single band at 52 kDa.