Enzymatic transformation in an electrochemical reactor utilizing a redox mediator-modified enzyme electrode for NAD(H) regeneration

Lipoamide dehydrogenase covalently modified with N-ethyl-N′-(γ-aminopropyl)-viologen bromide is dip-coated onto a carbon electrode under a Nafion® film. This provides a novel system for electroenzymatic regeneration of NADH which can be coupled to NADH-dependent enzyme-catalyzed synthesis.