Nitrogen transfer in E. coli glucosamine-6P synthase. Investigations using substrate and bisubstrate analogs

The compound 2-amino-2-deoxyglucitol-6P competitively inhibits E. coli glucosamine-6P synthase with respect to fructose-6P whereas glutamate γ-semialdehyde is a competitive inhibitor with respect to glutamine. These compounds, which exhibit good inhibitory properties (Km/Ki= 16 and 1000 respectively), were used in the synthesis of multisubstrate analogs.