Solid-phase synthesis of peptides containing O-phosphoryl serine and O-phosphoryl threonine using allyl group for phosphate protection

The preparation of Boc-Ser(PO3Allyl2)---OH and Boc-Thr(PO3Allyl2)---OH were described. Phosphorylated RB protein fragments were synthesized using these O-phosphorylated amino acid building blocks in the Boc mode of solid phase peptide synthesis. Abstract The preparation of Boc-Ser(PO3Allyl2)---OH and Boc-Thr(PO3Allyl2)---OH were described. Phosphorylated RB protein fragments were synthesized using these O-phosphorylated amino acid building blocks in the Boc mode of solid phase peptide synthesis.