Exploring the specificity of prenyl protein-specific methyltransferase with synthetic prenylated rab peptides

Most Rab proteins contain two C-terminal geranylgeranyl groups. Some members are C-terminally m methylated, whereas others are not. Synthetic peptides bearing the sequence of the C-termini of Rab proteins and containing the C-terminal motif C(S-geranylgeranyl)AC(S-geranylgeranyl) are substrates for the membrane-bound prenyl protein-specific methyltransferase, whereas those that end in C(S-geranylgeranyl)C(S-geranylgeranyl) are not. Abstract Most Rab proteins contain two C-terminal geranylgeranyl groups. Some members are C-terminally methylated, whereas others are not. Synthetic peptides bearing the sequence of the C-termini of Rab proteins and containing the C-terminal motif C(S-geranylgeranyl)AC(S-geranylgeranyl) are substrates for the membrane-bound prenyl protein-specific methyltransferase, whereas those that end in C(S-geranylgeranyl)C(S-geranylgeranyl) are not.