A PNA-DNA linker synthesis of N-((4,4′-dimethoxytrityloxy)ethyl)-N-(thymin-1-ylacetyl)glycine

N-(2-benzyloxyethyl)-N-(thymin-1-ylacetyl)glycine was synthesised and used as a linker for coupling DNA and PNA together. Three different 10-mers composed of 6 DNA units and 4 PNA thymine units were made. The PNA-DNA chimera hybridise to complementary DNA or PNA oligomers, and both the PNA and the DNA part of the chimera are involved in the binding.