Estimating KI values for tight binding inhibitors from dose-response plots

A simple method is described by which the Ki value of tight binding inhibitors of enzymes can be calculated directly from the IC50 value determined from graphical analysis of dose-response curves. Experimental verification of this method is provided by study of 15 inhibitors of the enzyme stromelysis which span a broad range of Ki values. A simple method is described by which the Ki value of tight binding inhibitors of enzymes can be calculated directly from the IC50 value determined from graphical analysis of dose-response curves. Experimental verification of this method is provided by study of 15 inhibitors of the enzyme stromelysis which span a broad range of Ki values.