Liquid chromatography mass spectrometry of antisense oligonucleotides

The development of rapid, simple and accurate methods for characterizing and insuring the integrity of oligonucleotides is important for the development of therapeutic and diagnostic products. This study was performed to determine the utility of high performance liquid chromatography (HPLC) with electrospray ionization mass spectrometry detection (LCMS) as an alternative or addition to the current techniques of HPLC, capillary electrophoresis and gel electrophoresis.